fetal human small intestinal fhs 74 int cells  (ATCC)

Journal: The Korean Journal of Parasitology

Article Title: Toxoplasma gondii Induces Apoptosis via Endoplasmic Reticulum Stress-Derived Mitochondrial Pathway in Human Small Intestinal Epithelial Cell-Line

doi: 10.3347/kjp.2021.59.6.573

Figure Lengend Snippet: Toxoplasma gondii and ESP induced cytotoxicity and apoptosis in human fetal small intestinal epithelial FHs 74 Int cells. FHs 74 Int cells were stimulated with the T. gondii RH strain at a multiplicity of infection (MOI) of 10 or with 1 μg/ml ESP for 0, 2, 8, and 24 hr. (A) The lactate dehydrogenase (LDH) level in the medium. The horizontal lines in each group represent mean±SD. *** P <0.001, compared to the untreated control cells. (B) Cleaved PARP and cleaved caspase 3 protein levels. (C, D) Expression of cytochrome c of in mitochondrial and cytosolic fractions.

Article Snippet: A nontransformed human fetal small intestinal epithelial cell-line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, Virginia, USA) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heat-inactivated fetal bovine serum (FBS), antibiotic-antimycotic (Gibco BRL) and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, New York, USA) at 37°C in a humidified atmosphere with 5% CO 2 .

Techniques: Infection, Control, Expressing

Journal: mSphere

Article Title: A Pilot Study To Establish an In Vitro Model To Study Premature Intestinal Epithelium and Gut Microbiota Interactions

doi: 10.1128/mSphere.00806-21

Figure Lengend Snippet: Differentially regulated gene ontology by late compared to early stool samples. The bar graphs show the degree of enrichment by gene ontology from epithelial cells exposed to the late stool samples in comparison to the exposure to the early stool samples. The differences increase from bottom to top. Immune system, development, and cell-cell adhesion genes are differentially regulated likely by changes in microbiome mass and composition. (A) Categories of genes that are downregulated in the late samples relative to the early samples. (B) Categories of genes that are upregulated in the late samples relative to the early samples. The names of the gene ontology groups are shown on the y axis, and the enrichment score is shown on the x axis. The shaded color of the bars indicates the P value for the significance test of enrichment. The statistics and GO accession numbers are included in Table S1 in the supplemental material.

Article Snippet: In this study, we exposed fetal epithelial cell cultures (FHs-74 int cells, human, ATCC CCL 241) to sterile fecal filtrates derived from stool collected from preterm infants at <2 and at 3 to 4 weeks of age.

Techniques: Comparison

Journal: mSphere

Article Title: A Pilot Study To Establish an In Vitro Model To Study Premature Intestinal Epithelium and Gut Microbiota Interactions

doi: 10.1128/mSphere.00806-21

Figure Lengend Snippet: Network graph of genus-gene interactions. The network graph shows the associations between bacterial genera from stool samples and gene expression from epithelial cells. The genera are in italics. Arrows point to genes, and the arrows indicate if the relationship is one of downregulation (blue) or upregulation (red). Genes that have been linked to apoptosis in the literature are in purple.

Article Snippet: In this study, we exposed fetal epithelial cell cultures (FHs-74 int cells, human, ATCC CCL 241) to sterile fecal filtrates derived from stool collected from preterm infants at <2 and at 3 to 4 weeks of age.

Techniques: Gene Expression

Journal: Parasites & Vectors

Article Title: Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii -infected human small intestinal epithelial cells

doi: 10.1186/s13071-021-04666-w

Figure Lengend Snippet: Expression of nucleotide-binding oligomerization domain-like receptors (NLRs), inflammasome components, and caspase-cleaved interleukins (ILs) in human small intestinal epithelial (FHs 74 Int) cells. Total RNA isolated from the untreated cells was examined by polymerase chain reaction (PCR) for mRNA expression of different genes. a Expression of NLR mRNAs in FHs 74 Int cells. b Expression of the NLR mRNAs in FHs 74 Int cells compared to HPRT-1. c Positive controls for primer functionality using human acute monocytic leukemia cell line (THP-1). d Inflammasome components and caspase-cleaved ILs expressed in FHs 74 Int cells. Images shown are representatives of five independent experiments

Article Snippet: A non-transformed human fetal small intestinal epithelial cell line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, VA, USA) and cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum (FBS), an antibiotic–antimycotic solution, and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere at 5% (v/v) CO 2 .

Techniques: Expressing, Binding Assay, Isolation, Polymerase Chain Reaction

Journal: Parasites & Vectors

Article Title: Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii -infected human small intestinal epithelial cells

doi: 10.1186/s13071-021-04666-w

Figure Lengend Snippet: Expression of NLR family members, NLRP1, NLRP3, NLRP6, and NLRC4 inflammasome components in response to T. gondii infection in human small intestinal epithelial (FHs 74 Int) cells. a FHs 74 Int cells were infected with T. gondii at a MOI of 10 for 0, 4, and 8 h. Expression of NLRP1, NLRP3, NLRP6, and NLRC4 inflammasome component proteins were detected by western blot analysis; α-tubulin was used as the loading control. b FHs 74 Int cells were fixed and probed against cleaved caspase-8 (green). The cells were counterstained with 4′,6-diamidino-2-phenylindole (blue) and visualized using confocal microscopy. All data shown are representative of three independent experiments with similar results

Article Snippet: A non-transformed human fetal small intestinal epithelial cell line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, VA, USA) and cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum (FBS), an antibiotic–antimycotic solution, and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere at 5% (v/v) CO 2 .

Techniques: Expressing, Infection, Western Blot, Control, Confocal Microscopy

Journal: Parasites & Vectors

Article Title: Expression profiles of NOD-like receptors and regulation of NLRP3 inflammasome activation in Toxoplasma gondii -infected human small intestinal epithelial cells

doi: 10.1186/s13071-021-04666-w

Figure Lengend Snippet: Schematic model of NLRP3 inflammasome activation in T. gondii -infected FHs 74 Int cells. T. gondii activates the p38 MAPK pathway in small intestinal epithelial cells, subsequently upregulating protein expression and promoting the formation of the NLRP3 inflammasome. The NLRP3 inflammasome cleaves pro-IL-1β, pro-IL-18 and pro-IL-33 to become active IL-1β, IL-18, and IL-33, which ultimately induces cytotoxicity of FHs 74 Int cells

Article Snippet: A non-transformed human fetal small intestinal epithelial cell line (FHs 74 Int cells) was purchased from ATCC (ATCC, Manassas, VA, USA) and cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum (FBS), an antibiotic–antimycotic solution, and 30 ng/ml human epidermal growth factor (all from Gibco, Grand Island, NY, USA) at 37 °C in a humidified atmosphere at 5% (v/v) CO 2 .

Techniques: Activation Assay, Infection, Expressing